Table 1 Bacterial strains and plasmids
From: Methylation of rRNA as a host defense against rampant group II intron retrotransposition
| Bacteria | Relevant characteristics; comments | Reference |
|---|---|---|
| L. lactis IL1403 | plasmid free; recipient strain, strain for all RTP assays; sequenced genome is available (GenBank: NC_002662) | [22] |
| E. coli DH5α | F− endAI recAl hsdRl7 (rK− mK−) deoR supE44 thi−J gyrA96 relA | Gibco-BRL |
| Plasmids | Relevant characteristics; comments | Reference |
| pLNRK-RIG | L. lactis/E. coli shuttle vector contains nisin-inducible promoter, nisR nisK, CamR, RIG cassette, intron donor | [21] |
| pGh4:ISS1 | pG + host replicon, thermosensitive (replicates at 28 °C but lost above 37 °C), ErmR | [23] |
| pRS01 | co-integrant of conjugative plasmid pRS01 and pTRK28, ErmR | [24] |
| pLNRK-GFP | Derived from pLNRK-RIG with green fluorescent protein (GFP) replacing the entire intron and RIG cassette. CamR | This study |
| pMN1343 | pMN1343 SpcR, contains intron homing site | [25] |
| pGh5: PnisA | pG + host replicon, ErmR, backbone of pGh5 from [23], with ISS1 removed, PnisA inserted. Used as empty vector control in the wild-type background in complementation assays. | [23] and this study |
| pGh5: PnisA rlmH | pGh5:PnisA with the rlmH gene inserted under control of the PnisA promoter. Used for complementation assays. | [23] and this study |
| pLNRK-SA | pLNRK smEx::Ll.LtrB ΔORF (SA)-nLIC; expression construct for Ll.LtrB intron with streptavidin aptamer in domain IVb, CamR | [26] |